Virus:Article Title: The Lateral Root Cap Acts as an Auxin Sink that Controls Meristem Size.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP Thermo-Fisher Cat# A6455; RRID:AB_221570 Rabbit polyclonal anti-endoplasmin/GRP94 [36] RRID:AB_10791076 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate Thermo-Fisher Cat# A16096; RRID:AB_2534770 Bacterial and Virus Strains Escherichia coli DH5a N/A N/A Agrobacterium tumefaciens GV3101 N/A N/A Chemicals, Peptides, and Recombinant Proteins Murashige & Skoog Medium Duchefa Cat# M0221 Plant-agar Duchefa Cat# P1001 Sucrose Duchefa Cat# S0809 Kanamycin Sigma-Aldrich Cat# K1377 Rifampicin Duchefa Cat# R0146 Tetracycline Duchefa Cat# T0150 Gentamicin Duchefa Cat# G0124 Streptomycin Duchefa Cat# S0148 Spectinomycin Duchefa Cat# S0188 Phosphinothricin Sigma-Aldrich Cat# 77182-82-2 Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530S trans-Zeatin Sigma-Aldrich Cat# Z0876 Dexamethasone Sigma-Aldrich Cat# D4902 Hpy188III NEB Cat# R0622S Complete protease inhibitor cocktail Roche Cat# 11697498001 Critical Commercial Assays SensiFAST SYBR Bioline Cat# BIO-92005 NucleoSpin RNA Plus Macherey-Nagel Cat# 740984 qPCRBIO SyGreen Mix PCR Biosystems Cat# PB20.11-05 Gel/PCR DNA Fragments Extraction Kit Geneaid Cat# DF100 NucleoSpin Plasmid Macherey-Nagel Cat# 740588 Gateway BP Clonase II Thermo-Fisher Cat# 11789 Gateway LR Clonase II Thermo-Fisher Cat# 11791 Superscript VILO cDNA Synthesis Kit Thermo-Fisher Cat# 11754 Rneasy Micro Kit QIAGEN Cat# 74004 Deposited Data Microarray experiments Gene Expression Omnibus GEO: GSE117424 Experimental Models: Organisms/Strains Arabidopsis: Col-0 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: Landsberg erecta Nottingham Arabidopsis Stock Centre N/A Arabidopsis: gh3.17-1 [10] N/A Arabidopsis: pin5-3 Nottingham Arabidopsis Stock Centre SALK_021738 Arabidopsis: shy2-31 [9] N/A Arabidopsis: UBQ10:GH3.17 [10] N/A (Continued on next page) e1 Current Biology 29, 1–7.e1–e4, April 1, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Arabidopsis: PIN5OX [26] N/A Arabidopsis: UAS:GH3.17-GR This paper N/A Arabidopsis: UAS:ARR1-GR This paper N/A Arabidopsis: UAS:CKX1-GR This paper N/A Arabidopsis: J2632 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: M0126 Nottingham Arabidopsis Stock Centre N/A Oligonucleotides See Methods section and Table S1 N/A N/A Recombinant DNA pB7m43GW [10] N/A P4P1-UAS This paper N/A P221-GH3.17 [10] N/A P2P3-GR This paper N/A pGreenII0229 - UAS::AtCKX1-GR This paper N/A pBI121 - UAS::ARR1DDDK-GR This paper N/A Software and Algorithms Excel Microsoft N/A ImageJ https://imagej.nih.gov/ij/ N/A ChipEnrich http://www.arexdb.org/software.html N/A VirtualPlant 1.0 http://virtualplant.bio.nyu.edu/cgi-bin/vpweb/ N/A TMeV http://www.tm4.org/ N/A Other Zeiss LSM 780 Zeiss N/A Fitotron SGC 120 Growth chamber Weiss Technik, UK N/A Zeiss Axio Imager A2 Zeiss N/A 7500 Fast Real-Time PCR system Applied Biosystems N/A Zen software Zeiss N/A
Article Title: A PHABULOSA-Controlled Genetic Pathway Regulates Ground Tissue Patterning in the Arabidopsis Root
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a N/A N/A Agrobacterium tumefaciens GV3101 N/A N/A Chemicals, Peptides, and Recombinant Proteins Murashige & Skoog Medium Duchefa Cat# M0221 MES hydrate Duchefa Cat# M1503 Plant-agar Duchefa Cat# P1001 Sucrose Duchefa Cat# S0809 Kanamycin Sigma-Aldrich Cat# K1377 Rifampicin Duchefa Cat# R0146 Tetracycline Duchefa Cat# T0150 Gentamicin Duchefa Cat# G0124 Streptomycin Duchefa Cat# S0148 Spectinomycin Duchefa Cat# S0188 Phosphinothricin Sigma-Aldrich Cat# 77182-82-2 Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530S trans-Zeatin Sigma-Aldrich Cat# Z0876 Dexamethasone Sigma-Aldrich Cat# D4902 Hpy188III NEB Cat# R0622S Complete protease inhibitor cocktail Roche Cat# 11697498001 x-GlcA Duchefa Cat# X1405.1000 Dimethyl-sulfoxide Sigma-Aldrich Cat# 67-68-5 Ethanol Sigma-Aldrich Cat# 64-17-5 Na2HPO4 Duchefa Cat# 10028-24-7 NaH2PO4 Carlo Erba Cat# 7558-80-7 K3 Fe(CN)6 Sigma-Aldrich Cat# 13746-66-2 K4Fe(CN)6 Sigma-Aldrich Cat# 14459-95-1 Chloral Hydrate Acros Organics Cat# 302-17-0 Glycerol Sigma-Aldrich Cat# 56-81-5 Paclobutrazol Duchefa Cat# P0922.0500 GA4+7 Duchefa-Biochemie Cat# G0938 Glycine BIORAD Cat# 1610718 Formaldehyde solution 37% Sigma-Aldrich Cat# 252549 Miracloth Merck-Millipore Cat# 475855 Hydrocloric acid 37% Fisher scientific Cat# 1298971 Magnesium chloride Carlo Erba Cat# 459337 DL-1,4-Dithiothreitol Acros Organics Cat# 327190100 Triton X-100 Acros Organics Cat# 215680010 Ethylenediaminetetraacetic acid disodium salt Carlo Erba Cat# 405497 Sodium lauryl sulfate Carlo Erba Cat# P7600517 Sodium chloride Duchefa-Biochemie Cat# S0520.5000 GFP Trap_A beads Chromotek Cat# 141205001A GFP-Trap Magnetic Agarose beads Chromotek Cat# 90312001MA Tween 20 Acros Organics Cat# 233362500 (Continued on next page) Current Biology 31, 420–426.e1–e6, January 25, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Proteinase K Invitrogen Cat# 1657252 Tris Ultrapure Duchefa-Biochemie Cat# 010894.04 Sodium acetate Carlo Erba Cat# 478137 Basic Fuchsine BioPlus Cat#2177006-1 Xylitol Sigma-Aldrich Cat# 87-99-0 Sodium deoxycholate Sigma-Aldrich Cat# 302-95-4 Urea Acros Organics Cat# 57-13-6 Propidium Iodide Sigma-Aldrich Cat# MKBV0241V Critical Commercial Assays SensiFAST SYBR Bioline Cat# BIO-92005 NucleoSpin RNA Plus Macherey-Nagel Cat# 740984 qPCRBIO SyGreen Mix PCR Biosystems Cat# PB20.11-05 Gel/PCR DNA Fragments Extraction Kit Geneaid Cat# DF100 NucleoSpin Plasmid Macherey-Nagel Cat# 740588 Gateway BP Clonase II Thermo-Fisher Cat# 11789 Gateway LR Clonase II Thermo-Fisher Cat# 11791 Superscript VILO cDNA Synthesis Kit Thermo-Fisher Cat# 11754 Rneasy Micro Kit QIAGEN Cat# 74004 MinElute Reaction Cleanup Kit (50) QIAGEN Cat# 28204b Experimental Models: Organisms/Strains Arabidopsis: Col-0 NASC N/A Arabidopsis: phb-13,phv-11 ER+ This paper N/A Arabidopsis: cycd6-1 NASC SALK_021738 Arabidopsis: CYCD6;1::GFP:GUS 5 N/A Arabidopsis: UAS::PHBmu 9 N/A Arabidopsis: Q0990 NASC N9217 Arabidopsis: gai-t6 39 N/A Arabidopsis: gai-1ER+,CYCD6;1::GFP:GUS This paper N/A Arabidoopsis: CO2::His2B:YFP 13 N/A Arabidopsis: gai-2 NASC SAIL_587_C02 Arabidopsis: gai-3 NASC SALK_208684 Arabidopis: GAI-GFP 40 N/A Arabidopsis: GA2ox2::GUS 41 N/A Arabidopsis: UAS:GA2ox2 This paper N/A Arabidopsis: UAS::MIM165/6 This paper N/A Arabidopsis: EN7::GAL4 This paper N/A Arabidopsis: phb-1d 12 N/A Arabidopsis: ga2ox2-1 NASC SALK_051749 Arabidopsis: RGA-GFP 32 N/A Arabidopsis: phb-1d,RGA-GFP This paper N/A Arabidopsis: phb-1d,GAI-GFP This paper N/A Arabidopsis: PHB::GFP 12 N/A Arabidopsis: PHB-GFP 12 N/A Arabidopsis: scl3-1 NASC SALK_002516 Arabidopsis: scl3-1,phb13,phv11 This paper N/A Arabidopsis: shr-2 42 CS2972 Arabidopsis: MIR165A::GFP 9 N/A Arabidopsis: MIR166A::GFP 9 N/A (Continued on next page) ll OPEN ACCESS e2 Current Biology 31, 420–426.e1–e6, January 25, 2021 Report
Recombinant:Article Title: The Lateral Root Cap Acts as an Auxin Sink that Controls Meristem Size.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP Thermo-Fisher Cat# A6455; RRID:AB_221570 Rabbit polyclonal anti-endoplasmin/GRP94 [36] RRID:AB_10791076 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate Thermo-Fisher Cat# A16096; RRID:AB_2534770 Bacterial and Virus Strains Escherichia coli DH5a N/A N/A Agrobacterium tumefaciens GV3101 N/A N/A Chemicals, Peptides, and Recombinant Proteins Murashige & Skoog Medium Duchefa Cat# M0221 Plant-agar Duchefa Cat# P1001 Sucrose Duchefa Cat# S0809 Kanamycin Sigma-Aldrich Cat# K1377 Rifampicin Duchefa Cat# R0146 Tetracycline Duchefa Cat# T0150 Gentamicin Duchefa Cat# G0124 Streptomycin Duchefa Cat# S0148 Spectinomycin Duchefa Cat# S0188 Phosphinothricin Sigma-Aldrich Cat# 77182-82-2 Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530S trans-Zeatin Sigma-Aldrich Cat# Z0876 Dexamethasone Sigma-Aldrich Cat# D4902 Hpy188III NEB Cat# R0622S Complete protease inhibitor cocktail Roche Cat# 11697498001 Critical Commercial Assays SensiFAST SYBR Bioline Cat# BIO-92005 NucleoSpin RNA Plus Macherey-Nagel Cat# 740984 qPCRBIO SyGreen Mix PCR Biosystems Cat# PB20.11-05 Gel/PCR DNA Fragments Extraction Kit Geneaid Cat# DF100 NucleoSpin Plasmid Macherey-Nagel Cat# 740588 Gateway BP Clonase II Thermo-Fisher Cat# 11789 Gateway LR Clonase II Thermo-Fisher Cat# 11791 Superscript VILO cDNA Synthesis Kit Thermo-Fisher Cat# 11754 Rneasy Micro Kit QIAGEN Cat# 74004 Deposited Data Microarray experiments Gene Expression Omnibus GEO: GSE117424 Experimental Models: Organisms/Strains Arabidopsis: Col-0 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: Landsberg erecta Nottingham Arabidopsis Stock Centre N/A Arabidopsis: gh3.17-1 [10] N/A Arabidopsis: pin5-3 Nottingham Arabidopsis Stock Centre SALK_021738 Arabidopsis: shy2-31 [9] N/A Arabidopsis: UBQ10:GH3.17 [10] N/A (Continued on next page) e1 Current Biology 29, 1–7.e1–e4, April 1, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Arabidopsis: PIN5OX [26] N/A Arabidopsis: UAS:GH3.17-GR This paper N/A Arabidopsis: UAS:ARR1-GR This paper N/A Arabidopsis: UAS:CKX1-GR This paper N/A Arabidopsis: J2632 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: M0126 Nottingham Arabidopsis Stock Centre N/A Oligonucleotides See Methods section and Table S1 N/A N/A Recombinant DNA pB7m43GW [10] N/A P4P1-UAS This paper N/A P221-GH3.17 [10] N/A P2P3-GR This paper N/A pGreenII0229 - UAS::AtCKX1-GR This paper N/A pBI121 - UAS::ARR1DDDK-GR This paper N/A Software and Algorithms Excel Microsoft N/A ImageJ https://imagej.nih.gov/ij/ N/A ChipEnrich http://www.arexdb.org/software.html N/A VirtualPlant 1.0 http://virtualplant.bio.nyu.edu/cgi-bin/vpweb/ N/A TMeV http://www.tm4.org/ N/A Other Zeiss LSM 780 Zeiss N/A Fitotron SGC 120 Growth chamber Weiss Technik, UK N/A Zeiss Axio Imager A2 Zeiss N/A 7500 Fast Real-Time PCR system Applied Biosystems N/A Zen software Zeiss N/A
Article Title: A PHABULOSA-Controlled Genetic Pathway Regulates Ground Tissue Patterning in the Arabidopsis Root
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a N/A N/A Agrobacterium tumefaciens GV3101 N/A N/A Chemicals, Peptides, and Recombinant Proteins Murashige & Skoog Medium Duchefa Cat# M0221 MES hydrate Duchefa Cat# M1503 Plant-agar Duchefa Cat# P1001 Sucrose Duchefa Cat# S0809 Kanamycin Sigma-Aldrich Cat# K1377 Rifampicin Duchefa Cat# R0146 Tetracycline Duchefa Cat# T0150 Gentamicin Duchefa Cat# G0124 Streptomycin Duchefa Cat# S0148 Spectinomycin Duchefa Cat# S0188 Phosphinothricin Sigma-Aldrich Cat# 77182-82-2 Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530S trans-Zeatin Sigma-Aldrich Cat# Z0876 Dexamethasone Sigma-Aldrich Cat# D4902 Hpy188III NEB Cat# R0622S Complete protease inhibitor cocktail Roche Cat# 11697498001 x-GlcA Duchefa Cat# X1405.1000 Dimethyl-sulfoxide Sigma-Aldrich Cat# 67-68-5 Ethanol Sigma-Aldrich Cat# 64-17-5 Na2HPO4 Duchefa Cat# 10028-24-7 NaH2PO4 Carlo Erba Cat# 7558-80-7 K3 Fe(CN)6 Sigma-Aldrich Cat# 13746-66-2 K4Fe(CN)6 Sigma-Aldrich Cat# 14459-95-1 Chloral Hydrate Acros Organics Cat# 302-17-0 Glycerol Sigma-Aldrich Cat# 56-81-5 Paclobutrazol Duchefa Cat# P0922.0500 GA4+7 Duchefa-Biochemie Cat# G0938 Glycine BIORAD Cat# 1610718 Formaldehyde solution 37% Sigma-Aldrich Cat# 252549 Miracloth Merck-Millipore Cat# 475855 Hydrocloric acid 37% Fisher scientific Cat# 1298971 Magnesium chloride Carlo Erba Cat# 459337 DL-1,4-Dithiothreitol Acros Organics Cat# 327190100 Triton X-100 Acros Organics Cat# 215680010 Ethylenediaminetetraacetic acid disodium salt Carlo Erba Cat# 405497 Sodium lauryl sulfate Carlo Erba Cat# P7600517 Sodium chloride Duchefa-Biochemie Cat# S0520.5000 GFP Trap_A beads Chromotek Cat# 141205001A GFP-Trap Magnetic Agarose beads Chromotek Cat# 90312001MA Tween 20 Acros Organics Cat# 233362500 (Continued on next page) Current Biology 31, 420–426.e1–e6, January 25, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Proteinase K Invitrogen Cat# 1657252 Tris Ultrapure Duchefa-Biochemie Cat# 010894.04 Sodium acetate Carlo Erba Cat# 478137 Basic Fuchsine BioPlus Cat#2177006-1 Xylitol Sigma-Aldrich Cat# 87-99-0 Sodium deoxycholate Sigma-Aldrich Cat# 302-95-4 Urea Acros Organics Cat# 57-13-6 Propidium Iodide Sigma-Aldrich Cat# MKBV0241V Critical Commercial Assays SensiFAST SYBR Bioline Cat# BIO-92005 NucleoSpin RNA Plus Macherey-Nagel Cat# 740984 qPCRBIO SyGreen Mix PCR Biosystems Cat# PB20.11-05 Gel/PCR DNA Fragments Extraction Kit Geneaid Cat# DF100 NucleoSpin Plasmid Macherey-Nagel Cat# 740588 Gateway BP Clonase II Thermo-Fisher Cat# 11789 Gateway LR Clonase II Thermo-Fisher Cat# 11791 Superscript VILO cDNA Synthesis Kit Thermo-Fisher Cat# 11754 Rneasy Micro Kit QIAGEN Cat# 74004 MinElute Reaction Cleanup Kit (50) QIAGEN Cat# 28204b Experimental Models: Organisms/Strains Arabidopsis: Col-0 NASC N/A Arabidopsis: phb-13,phv-11 ER+ This paper N/A Arabidopsis: cycd6-1 NASC SALK_021738 Arabidopsis: CYCD6;1::GFP:GUS 5 N/A Arabidopsis: UAS::PHBmu 9 N/A Arabidopsis: Q0990 NASC N9217 Arabidopsis: gai-t6 39 N/A Arabidopsis: gai-1ER+,CYCD6;1::GFP:GUS This paper N/A Arabidoopsis: CO2::His2B:YFP 13 N/A Arabidopsis: gai-2 NASC SAIL_587_C02 Arabidopsis: gai-3 NASC SALK_208684 Arabidopis: GAI-GFP 40 N/A Arabidopsis: GA2ox2::GUS 41 N/A Arabidopsis: UAS:GA2ox2 This paper N/A Arabidopsis: UAS::MIM165/6 This paper N/A Arabidopsis: EN7::GAL4 This paper N/A Arabidopsis: phb-1d 12 N/A Arabidopsis: ga2ox2-1 NASC SALK_051749 Arabidopsis: RGA-GFP 32 N/A Arabidopsis: phb-1d,RGA-GFP This paper N/A Arabidopsis: phb-1d,GAI-GFP This paper N/A Arabidopsis: PHB::GFP 12 N/A Arabidopsis: PHB-GFP 12 N/A Arabidopsis: scl3-1 NASC SALK_002516 Arabidopsis: scl3-1,phb13,phv11 This paper N/A Arabidopsis: shr-2 42 CS2972 Arabidopsis: MIR165A::GFP 9 N/A Arabidopsis: MIR166A::GFP 9 N/A (Continued on next page) ll OPEN ACCESS e2 Current Biology 31, 420–426.e1–e6, January 25, 2021 Report
Protease Inhibitor:Article Title: The Lateral Root Cap Acts as an Auxin Sink that Controls Meristem Size.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP Thermo-Fisher Cat# A6455; RRID:AB_221570 Rabbit polyclonal anti-endoplasmin/GRP94 [36] RRID:AB_10791076 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate Thermo-Fisher Cat# A16096; RRID:AB_2534770 Bacterial and Virus Strains Escherichia coli DH5a N/A N/A Agrobacterium tumefaciens GV3101 N/A N/A Chemicals, Peptides, and Recombinant Proteins Murashige & Skoog Medium Duchefa Cat# M0221 Plant-agar Duchefa Cat# P1001 Sucrose Duchefa Cat# S0809 Kanamycin Sigma-Aldrich Cat# K1377 Rifampicin Duchefa Cat# R0146 Tetracycline Duchefa Cat# T0150 Gentamicin Duchefa Cat# G0124 Streptomycin Duchefa Cat# S0148 Spectinomycin Duchefa Cat# S0188 Phosphinothricin Sigma-Aldrich Cat# 77182-82-2 Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530S trans-Zeatin Sigma-Aldrich Cat# Z0876 Dexamethasone Sigma-Aldrich Cat# D4902 Hpy188III NEB Cat# R0622S Complete protease inhibitor cocktail Roche Cat# 11697498001 Critical Commercial Assays SensiFAST SYBR Bioline Cat# BIO-92005 NucleoSpin RNA Plus Macherey-Nagel Cat# 740984 qPCRBIO SyGreen Mix PCR Biosystems Cat# PB20.11-05 Gel/PCR DNA Fragments Extraction Kit Geneaid Cat# DF100 NucleoSpin Plasmid Macherey-Nagel Cat# 740588 Gateway BP Clonase II Thermo-Fisher Cat# 11789 Gateway LR Clonase II Thermo-Fisher Cat# 11791 Superscript VILO cDNA Synthesis Kit Thermo-Fisher Cat# 11754 Rneasy Micro Kit QIAGEN Cat# 74004 Deposited Data Microarray experiments Gene Expression Omnibus GEO: GSE117424 Experimental Models: Organisms/Strains Arabidopsis: Col-0 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: Landsberg erecta Nottingham Arabidopsis Stock Centre N/A Arabidopsis: gh3.17-1 [10] N/A Arabidopsis: pin5-3 Nottingham Arabidopsis Stock Centre SALK_021738 Arabidopsis: shy2-31 [9] N/A Arabidopsis: UBQ10:GH3.17 [10] N/A (Continued on next page) e1 Current Biology 29, 1–7.e1–e4, April 1, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Arabidopsis: PIN5OX [26] N/A Arabidopsis: UAS:GH3.17-GR This paper N/A Arabidopsis: UAS:ARR1-GR This paper N/A Arabidopsis: UAS:CKX1-GR This paper N/A Arabidopsis: J2632 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: M0126 Nottingham Arabidopsis Stock Centre N/A Oligonucleotides See Methods section and Table S1 N/A N/A Recombinant DNA pB7m43GW [10] N/A P4P1-UAS This paper N/A P221-GH3.17 [10] N/A P2P3-GR This paper N/A pGreenII0229 - UAS::AtCKX1-GR This paper N/A pBI121 - UAS::ARR1DDDK-GR This paper N/A Software and Algorithms Excel Microsoft N/A ImageJ https://imagej.nih.gov/ij/ N/A ChipEnrich http://www.arexdb.org/software.html N/A VirtualPlant 1.0 http://virtualplant.bio.nyu.edu/cgi-bin/vpweb/ N/A TMeV http://www.tm4.org/ N/A Other Zeiss LSM 780 Zeiss N/A Fitotron SGC 120 Growth chamber Weiss Technik, UK N/A Zeiss Axio Imager A2 Zeiss N/A 7500 Fast Real-Time PCR system Applied Biosystems N/A Zen software Zeiss N/A
Article Title: A PHABULOSA-Controlled Genetic Pathway Regulates Ground Tissue Patterning in the Arabidopsis Root
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a N/A N/A Agrobacterium tumefaciens GV3101 N/A N/A Chemicals, Peptides, and Recombinant Proteins Murashige & Skoog Medium Duchefa Cat# M0221 MES hydrate Duchefa Cat# M1503 Plant-agar Duchefa Cat# P1001 Sucrose Duchefa Cat# S0809 Kanamycin Sigma-Aldrich Cat# K1377 Rifampicin Duchefa Cat# R0146 Tetracycline Duchefa Cat# T0150 Gentamicin Duchefa Cat# G0124 Streptomycin Duchefa Cat# S0148 Spectinomycin Duchefa Cat# S0188 Phosphinothricin Sigma-Aldrich Cat# 77182-82-2 Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530S trans-Zeatin Sigma-Aldrich Cat# Z0876 Dexamethasone Sigma-Aldrich Cat# D4902 Hpy188III NEB Cat# R0622S Complete protease inhibitor cocktail Roche Cat# 11697498001 x-GlcA Duchefa Cat# X1405.1000 Dimethyl-sulfoxide Sigma-Aldrich Cat# 67-68-5 Ethanol Sigma-Aldrich Cat# 64-17-5 Na2HPO4 Duchefa Cat# 10028-24-7 NaH2PO4 Carlo Erba Cat# 7558-80-7 K3 Fe(CN)6 Sigma-Aldrich Cat# 13746-66-2 K4Fe(CN)6 Sigma-Aldrich Cat# 14459-95-1 Chloral Hydrate Acros Organics Cat# 302-17-0 Glycerol Sigma-Aldrich Cat# 56-81-5 Paclobutrazol Duchefa Cat# P0922.0500 GA4+7 Duchefa-Biochemie Cat# G0938 Glycine BIORAD Cat# 1610718 Formaldehyde solution 37% Sigma-Aldrich Cat# 252549 Miracloth Merck-Millipore Cat# 475855 Hydrocloric acid 37% Fisher scientific Cat# 1298971 Magnesium chloride Carlo Erba Cat# 459337 DL-1,4-Dithiothreitol Acros Organics Cat# 327190100 Triton X-100 Acros Organics Cat# 215680010 Ethylenediaminetetraacetic acid disodium salt Carlo Erba Cat# 405497 Sodium lauryl sulfate Carlo Erba Cat# P7600517 Sodium chloride Duchefa-Biochemie Cat# S0520.5000 GFP Trap_A beads Chromotek Cat# 141205001A GFP-Trap Magnetic Agarose beads Chromotek Cat# 90312001MA Tween 20 Acros Organics Cat# 233362500 (Continued on next page) Current Biology 31, 420–426.e1–e6, January 25, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Proteinase K Invitrogen Cat# 1657252 Tris Ultrapure Duchefa-Biochemie Cat# 010894.04 Sodium acetate Carlo Erba Cat# 478137 Basic Fuchsine BioPlus Cat#2177006-1 Xylitol Sigma-Aldrich Cat# 87-99-0 Sodium deoxycholate Sigma-Aldrich Cat# 302-95-4 Urea Acros Organics Cat# 57-13-6 Propidium Iodide Sigma-Aldrich Cat# MKBV0241V Critical Commercial Assays SensiFAST SYBR Bioline Cat# BIO-92005 NucleoSpin RNA Plus Macherey-Nagel Cat# 740984 qPCRBIO SyGreen Mix PCR Biosystems Cat# PB20.11-05 Gel/PCR DNA Fragments Extraction Kit Geneaid Cat# DF100 NucleoSpin Plasmid Macherey-Nagel Cat# 740588 Gateway BP Clonase II Thermo-Fisher Cat# 11789 Gateway LR Clonase II Thermo-Fisher Cat# 11791 Superscript VILO cDNA Synthesis Kit Thermo-Fisher Cat# 11754 Rneasy Micro Kit QIAGEN Cat# 74004 MinElute Reaction Cleanup Kit (50) QIAGEN Cat# 28204b Experimental Models: Organisms/Strains Arabidopsis: Col-0 NASC N/A Arabidopsis: phb-13,phv-11 ER+ This paper N/A Arabidopsis: cycd6-1 NASC SALK_021738 Arabidopsis: CYCD6;1::GFP:GUS 5 N/A Arabidopsis: UAS::PHBmu 9 N/A Arabidopsis: Q0990 NASC N9217 Arabidopsis: gai-t6 39 N/A Arabidopsis: gai-1ER+,CYCD6;1::GFP:GUS This paper N/A Arabidoopsis: CO2::His2B:YFP 13 N/A Arabidopsis: gai-2 NASC SAIL_587_C02 Arabidopsis: gai-3 NASC SALK_208684 Arabidopis: GAI-GFP 40 N/A Arabidopsis: GA2ox2::GUS 41 N/A Arabidopsis: UAS:GA2ox2 This paper N/A Arabidopsis: UAS::MIM165/6 This paper N/A Arabidopsis: EN7::GAL4 This paper N/A Arabidopsis: phb-1d 12 N/A Arabidopsis: ga2ox2-1 NASC SALK_051749 Arabidopsis: RGA-GFP 32 N/A Arabidopsis: phb-1d,RGA-GFP This paper N/A Arabidopsis: phb-1d,GAI-GFP This paper N/A Arabidopsis: PHB::GFP 12 N/A Arabidopsis: PHB-GFP 12 N/A Arabidopsis: scl3-1 NASC SALK_002516 Arabidopsis: scl3-1,phb13,phv11 This paper N/A Arabidopsis: shr-2 42 CS2972 Arabidopsis: MIR165A::GFP 9 N/A Arabidopsis: MIR166A::GFP 9 N/A (Continued on next page) ll OPEN ACCESS e2 Current Biology 31, 420–426.e1–e6, January 25, 2021 Report
Extraction:Article Title: The Lateral Root Cap Acts as an Auxin Sink that Controls Meristem Size.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP Thermo-Fisher Cat# A6455; RRID:AB_221570 Rabbit polyclonal anti-endoplasmin/GRP94 [36] RRID:AB_10791076 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate Thermo-Fisher Cat# A16096; RRID:AB_2534770 Bacterial and Virus Strains Escherichia coli DH5a N/A N/A Agrobacterium tumefaciens GV3101 N/A N/A Chemicals, Peptides, and Recombinant Proteins Murashige & Skoog Medium Duchefa Cat# M0221 Plant-agar Duchefa Cat# P1001 Sucrose Duchefa Cat# S0809 Kanamycin Sigma-Aldrich Cat# K1377 Rifampicin Duchefa Cat# R0146 Tetracycline Duchefa Cat# T0150 Gentamicin Duchefa Cat# G0124 Streptomycin Duchefa Cat# S0148 Spectinomycin Duchefa Cat# S0188 Phosphinothricin Sigma-Aldrich Cat# 77182-82-2 Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530S trans-Zeatin Sigma-Aldrich Cat# Z0876 Dexamethasone Sigma-Aldrich Cat# D4902 Hpy188III NEB Cat# R0622S Complete protease inhibitor cocktail Roche Cat# 11697498001 Critical Commercial Assays SensiFAST SYBR Bioline Cat# BIO-92005 NucleoSpin RNA Plus Macherey-Nagel Cat# 740984 qPCRBIO SyGreen Mix PCR Biosystems Cat# PB20.11-05 Gel/PCR DNA Fragments Extraction Kit Geneaid Cat# DF100 NucleoSpin Plasmid Macherey-Nagel Cat# 740588 Gateway BP Clonase II Thermo-Fisher Cat# 11789 Gateway LR Clonase II Thermo-Fisher Cat# 11791 Superscript VILO cDNA Synthesis Kit Thermo-Fisher Cat# 11754 Rneasy Micro Kit QIAGEN Cat# 74004 Deposited Data Microarray experiments Gene Expression Omnibus GEO: GSE117424 Experimental Models: Organisms/Strains Arabidopsis: Col-0 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: Landsberg erecta Nottingham Arabidopsis Stock Centre N/A Arabidopsis: gh3.17-1 [10] N/A Arabidopsis: pin5-3 Nottingham Arabidopsis Stock Centre SALK_021738 Arabidopsis: shy2-31 [9] N/A Arabidopsis: UBQ10:GH3.17 [10] N/A (Continued on next page) e1 Current Biology 29, 1–7.e1–e4, April 1, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Arabidopsis: PIN5OX [26] N/A Arabidopsis: UAS:GH3.17-GR This paper N/A Arabidopsis: UAS:ARR1-GR This paper N/A Arabidopsis: UAS:CKX1-GR This paper N/A Arabidopsis: J2632 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: M0126 Nottingham Arabidopsis Stock Centre N/A Oligonucleotides See Methods section and Table S1 N/A N/A Recombinant DNA pB7m43GW [10] N/A P4P1-UAS This paper N/A P221-GH3.17 [10] N/A P2P3-GR This paper N/A pGreenII0229 - UAS::AtCKX1-GR This paper N/A pBI121 - UAS::ARR1DDDK-GR This paper N/A Software and Algorithms Excel Microsoft N/A ImageJ https://imagej.nih.gov/ij/ N/A ChipEnrich http://www.arexdb.org/software.html N/A VirtualPlant 1.0 http://virtualplant.bio.nyu.edu/cgi-bin/vpweb/ N/A TMeV http://www.tm4.org/ N/A Other Zeiss LSM 780 Zeiss N/A Fitotron SGC 120 Growth chamber Weiss Technik, UK N/A Zeiss Axio Imager A2 Zeiss N/A 7500 Fast Real-Time PCR system Applied Biosystems N/A Zen software Zeiss N/A
Plasmid Preparation:Article Title: The Lateral Root Cap Acts as an Auxin Sink that Controls Meristem Size.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP Thermo-Fisher Cat# A6455; RRID:AB_221570 Rabbit polyclonal anti-endoplasmin/GRP94 [36] RRID:AB_10791076 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate Thermo-Fisher Cat# A16096; RRID:AB_2534770 Bacterial and Virus Strains Escherichia coli DH5a N/A N/A Agrobacterium tumefaciens GV3101 N/A N/A Chemicals, Peptides, and Recombinant Proteins Murashige & Skoog Medium Duchefa Cat# M0221 Plant-agar Duchefa Cat# P1001 Sucrose Duchefa Cat# S0809 Kanamycin Sigma-Aldrich Cat# K1377 Rifampicin Duchefa Cat# R0146 Tetracycline Duchefa Cat# T0150 Gentamicin Duchefa Cat# G0124 Streptomycin Duchefa Cat# S0148 Spectinomycin Duchefa Cat# S0188 Phosphinothricin Sigma-Aldrich Cat# 77182-82-2 Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530S trans-Zeatin Sigma-Aldrich Cat# Z0876 Dexamethasone Sigma-Aldrich Cat# D4902 Hpy188III NEB Cat# R0622S Complete protease inhibitor cocktail Roche Cat# 11697498001 Critical Commercial Assays SensiFAST SYBR Bioline Cat# BIO-92005 NucleoSpin RNA Plus Macherey-Nagel Cat# 740984 qPCRBIO SyGreen Mix PCR Biosystems Cat# PB20.11-05 Gel/PCR DNA Fragments Extraction Kit Geneaid Cat# DF100 NucleoSpin Plasmid Macherey-Nagel Cat# 740588 Gateway BP Clonase II Thermo-Fisher Cat# 11789 Gateway LR Clonase II Thermo-Fisher Cat# 11791 Superscript VILO cDNA Synthesis Kit Thermo-Fisher Cat# 11754 Rneasy Micro Kit QIAGEN Cat# 74004 Deposited Data Microarray experiments Gene Expression Omnibus GEO: GSE117424 Experimental Models: Organisms/Strains Arabidopsis: Col-0 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: Landsberg erecta Nottingham Arabidopsis Stock Centre N/A Arabidopsis: gh3.17-1 [10] N/A Arabidopsis: pin5-3 Nottingham Arabidopsis Stock Centre SALK_021738 Arabidopsis: shy2-31 [9] N/A Arabidopsis: UBQ10:GH3.17 [10] N/A (Continued on next page) e1 Current Biology 29, 1–7.e1–e4, April 1, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Arabidopsis: PIN5OX [26] N/A Arabidopsis: UAS:GH3.17-GR This paper N/A Arabidopsis: UAS:ARR1-GR This paper N/A Arabidopsis: UAS:CKX1-GR This paper N/A Arabidopsis: J2632 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: M0126 Nottingham Arabidopsis Stock Centre N/A Oligonucleotides See Methods section and Table S1 N/A N/A Recombinant DNA pB7m43GW [10] N/A P4P1-UAS This paper N/A P221-GH3.17 [10] N/A P2P3-GR This paper N/A pGreenII0229 - UAS::AtCKX1-GR This paper N/A pBI121 - UAS::ARR1DDDK-GR This paper N/A Software and Algorithms Excel Microsoft N/A ImageJ https://imagej.nih.gov/ij/ N/A ChipEnrich http://www.arexdb.org/software.html N/A VirtualPlant 1.0 http://virtualplant.bio.nyu.edu/cgi-bin/vpweb/ N/A TMeV http://www.tm4.org/ N/A Other Zeiss LSM 780 Zeiss N/A Fitotron SGC 120 Growth chamber Weiss Technik, UK N/A Zeiss Axio Imager A2 Zeiss N/A 7500 Fast Real-Time PCR system Applied Biosystems N/A Zen software Zeiss N/A
cDNA Synthesis:Article Title: The Lateral Root Cap Acts as an Auxin Sink that Controls Meristem Size.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP Thermo-Fisher Cat# A6455; RRID:AB_221570 Rabbit polyclonal anti-endoplasmin/GRP94 [36] RRID:AB_10791076 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate Thermo-Fisher Cat# A16096; RRID:AB_2534770 Bacterial and Virus Strains Escherichia coli DH5a N/A N/A Agrobacterium tumefaciens GV3101 N/A N/A Chemicals, Peptides, and Recombinant Proteins Murashige & Skoog Medium Duchefa Cat# M0221 Plant-agar Duchefa Cat# P1001 Sucrose Duchefa Cat# S0809 Kanamycin Sigma-Aldrich Cat# K1377 Rifampicin Duchefa Cat# R0146 Tetracycline Duchefa Cat# T0150 Gentamicin Duchefa Cat# G0124 Streptomycin Duchefa Cat# S0148 Spectinomycin Duchefa Cat# S0188 Phosphinothricin Sigma-Aldrich Cat# 77182-82-2 Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530S trans-Zeatin Sigma-Aldrich Cat# Z0876 Dexamethasone Sigma-Aldrich Cat# D4902 Hpy188III NEB Cat# R0622S Complete protease inhibitor cocktail Roche Cat# 11697498001 Critical Commercial Assays SensiFAST SYBR Bioline Cat# BIO-92005 NucleoSpin RNA Plus Macherey-Nagel Cat# 740984 qPCRBIO SyGreen Mix PCR Biosystems Cat# PB20.11-05 Gel/PCR DNA Fragments Extraction Kit Geneaid Cat# DF100 NucleoSpin Plasmid Macherey-Nagel Cat# 740588 Gateway BP Clonase II Thermo-Fisher Cat# 11789 Gateway LR Clonase II Thermo-Fisher Cat# 11791 Superscript VILO cDNA Synthesis Kit Thermo-Fisher Cat# 11754 Rneasy Micro Kit QIAGEN Cat# 74004 Deposited Data Microarray experiments Gene Expression Omnibus GEO: GSE117424 Experimental Models: Organisms/Strains Arabidopsis: Col-0 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: Landsberg erecta Nottingham Arabidopsis Stock Centre N/A Arabidopsis: gh3.17-1 [10] N/A Arabidopsis: pin5-3 Nottingham Arabidopsis Stock Centre SALK_021738 Arabidopsis: shy2-31 [9] N/A Arabidopsis: UBQ10:GH3.17 [10] N/A (Continued on next page) e1 Current Biology 29, 1–7.e1–e4, April 1, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Arabidopsis: PIN5OX [26] N/A Arabidopsis: UAS:GH3.17-GR This paper N/A Arabidopsis: UAS:ARR1-GR This paper N/A Arabidopsis: UAS:CKX1-GR This paper N/A Arabidopsis: J2632 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: M0126 Nottingham Arabidopsis Stock Centre N/A Oligonucleotides See Methods section and Table S1 N/A N/A Recombinant DNA pB7m43GW [10] N/A P4P1-UAS This paper N/A P221-GH3.17 [10] N/A P2P3-GR This paper N/A pGreenII0229 - UAS::AtCKX1-GR This paper N/A pBI121 - UAS::ARR1DDDK-GR This paper N/A Software and Algorithms Excel Microsoft N/A ImageJ https://imagej.nih.gov/ij/ N/A ChipEnrich http://www.arexdb.org/software.html N/A VirtualPlant 1.0 http://virtualplant.bio.nyu.edu/cgi-bin/vpweb/ N/A TMeV http://www.tm4.org/ N/A Other Zeiss LSM 780 Zeiss N/A Fitotron SGC 120 Growth chamber Weiss Technik, UK N/A Zeiss Axio Imager A2 Zeiss N/A 7500 Fast Real-Time PCR system Applied Biosystems N/A Zen software Zeiss N/A
Microarray:Article Title: The Lateral Root Cap Acts as an Auxin Sink that Controls Meristem Size.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP Thermo-Fisher Cat# A6455; RRID:AB_221570 Rabbit polyclonal anti-endoplasmin/GRP94 [36] RRID:AB_10791076 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate Thermo-Fisher Cat# A16096; RRID:AB_2534770 Bacterial and Virus Strains Escherichia coli DH5a N/A N/A Agrobacterium tumefaciens GV3101 N/A N/A Chemicals, Peptides, and Recombinant Proteins Murashige & Skoog Medium Duchefa Cat# M0221 Plant-agar Duchefa Cat# P1001 Sucrose Duchefa Cat# S0809 Kanamycin Sigma-Aldrich Cat# K1377 Rifampicin Duchefa Cat# R0146 Tetracycline Duchefa Cat# T0150 Gentamicin Duchefa Cat# G0124 Streptomycin Duchefa Cat# S0148 Spectinomycin Duchefa Cat# S0188 Phosphinothricin Sigma-Aldrich Cat# 77182-82-2 Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530S trans-Zeatin Sigma-Aldrich Cat# Z0876 Dexamethasone Sigma-Aldrich Cat# D4902 Hpy188III NEB Cat# R0622S Complete protease inhibitor cocktail Roche Cat# 11697498001 Critical Commercial Assays SensiFAST SYBR Bioline Cat# BIO-92005 NucleoSpin RNA Plus Macherey-Nagel Cat# 740984 qPCRBIO SyGreen Mix PCR Biosystems Cat# PB20.11-05 Gel/PCR DNA Fragments Extraction Kit Geneaid Cat# DF100 NucleoSpin Plasmid Macherey-Nagel Cat# 740588 Gateway BP Clonase II Thermo-Fisher Cat# 11789 Gateway LR Clonase II Thermo-Fisher Cat# 11791 Superscript VILO cDNA Synthesis Kit Thermo-Fisher Cat# 11754 Rneasy Micro Kit QIAGEN Cat# 74004 Deposited Data Microarray experiments Gene Expression Omnibus GEO: GSE117424 Experimental Models: Organisms/Strains Arabidopsis: Col-0 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: Landsberg erecta Nottingham Arabidopsis Stock Centre N/A Arabidopsis: gh3.17-1 [10] N/A Arabidopsis: pin5-3 Nottingham Arabidopsis Stock Centre SALK_021738 Arabidopsis: shy2-31 [9] N/A Arabidopsis: UBQ10:GH3.17 [10] N/A (Continued on next page) e1 Current Biology 29, 1–7.e1–e4, April 1, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Arabidopsis: PIN5OX [26] N/A Arabidopsis: UAS:GH3.17-GR This paper N/A Arabidopsis: UAS:ARR1-GR This paper N/A Arabidopsis: UAS:CKX1-GR This paper N/A Arabidopsis: J2632 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: M0126 Nottingham Arabidopsis Stock Centre N/A Oligonucleotides See Methods section and Table S1 N/A N/A Recombinant DNA pB7m43GW [10] N/A P4P1-UAS This paper N/A P221-GH3.17 [10] N/A P2P3-GR This paper N/A pGreenII0229 - UAS::AtCKX1-GR This paper N/A pBI121 - UAS::ARR1DDDK-GR This paper N/A Software and Algorithms Excel Microsoft N/A ImageJ https://imagej.nih.gov/ij/ N/A ChipEnrich http://www.arexdb.org/software.html N/A VirtualPlant 1.0 http://virtualplant.bio.nyu.edu/cgi-bin/vpweb/ N/A TMeV http://www.tm4.org/ N/A Other Zeiss LSM 780 Zeiss N/A Fitotron SGC 120 Growth chamber Weiss Technik, UK N/A Zeiss Axio Imager A2 Zeiss N/A 7500 Fast Real-Time PCR system Applied Biosystems N/A Zen software Zeiss N/A
Gene Expression:Article Title: The Lateral Root Cap Acts as an Auxin Sink that Controls Meristem Size.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP Thermo-Fisher Cat# A6455; RRID:AB_221570 Rabbit polyclonal anti-endoplasmin/GRP94 [36] RRID:AB_10791076 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate Thermo-Fisher Cat# A16096; RRID:AB_2534770 Bacterial and Virus Strains Escherichia coli DH5a N/A N/A Agrobacterium tumefaciens GV3101 N/A N/A Chemicals, Peptides, and Recombinant Proteins Murashige & Skoog Medium Duchefa Cat# M0221 Plant-agar Duchefa Cat# P1001 Sucrose Duchefa Cat# S0809 Kanamycin Sigma-Aldrich Cat# K1377 Rifampicin Duchefa Cat# R0146 Tetracycline Duchefa Cat# T0150 Gentamicin Duchefa Cat# G0124 Streptomycin Duchefa Cat# S0148 Spectinomycin Duchefa Cat# S0188 Phosphinothricin Sigma-Aldrich Cat# 77182-82-2 Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530S trans-Zeatin Sigma-Aldrich Cat# Z0876 Dexamethasone Sigma-Aldrich Cat# D4902 Hpy188III NEB Cat# R0622S Complete protease inhibitor cocktail Roche Cat# 11697498001 Critical Commercial Assays SensiFAST SYBR Bioline Cat# BIO-92005 NucleoSpin RNA Plus Macherey-Nagel Cat# 740984 qPCRBIO SyGreen Mix PCR Biosystems Cat# PB20.11-05 Gel/PCR DNA Fragments Extraction Kit Geneaid Cat# DF100 NucleoSpin Plasmid Macherey-Nagel Cat# 740588 Gateway BP Clonase II Thermo-Fisher Cat# 11789 Gateway LR Clonase II Thermo-Fisher Cat# 11791 Superscript VILO cDNA Synthesis Kit Thermo-Fisher Cat# 11754 Rneasy Micro Kit QIAGEN Cat# 74004 Deposited Data Microarray experiments Gene Expression Omnibus GEO: GSE117424 Experimental Models: Organisms/Strains Arabidopsis: Col-0 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: Landsberg erecta Nottingham Arabidopsis Stock Centre N/A Arabidopsis: gh3.17-1 [10] N/A Arabidopsis: pin5-3 Nottingham Arabidopsis Stock Centre SALK_021738 Arabidopsis: shy2-31 [9] N/A Arabidopsis: UBQ10:GH3.17 [10] N/A (Continued on next page) e1 Current Biology 29, 1–7.e1–e4, April 1, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Arabidopsis: PIN5OX [26] N/A Arabidopsis: UAS:GH3.17-GR This paper N/A Arabidopsis: UAS:ARR1-GR This paper N/A Arabidopsis: UAS:CKX1-GR This paper N/A Arabidopsis: J2632 Nottingham Arabidopsis Stock Centre N/A Arabidopsis: M0126 Nottingham Arabidopsis Stock Centre N/A Oligonucleotides See Methods section and Table S1 N/A N/A Recombinant DNA pB7m43GW [10] N/A P4P1-UAS This paper N/A P221-GH3.17 [10] N/A P2P3-GR This paper N/A pGreenII0229 - UAS::AtCKX1-GR This paper N/A pBI121 - UAS::ARR1DDDK-GR This paper N/A Software and Algorithms Excel Microsoft N/A ImageJ https://imagej.nih.gov/ij/ N/A ChipEnrich http://www.arexdb.org/software.html N/A VirtualPlant 1.0 http://virtualplant.bio.nyu.edu/cgi-bin/vpweb/ N/A TMeV http://www.tm4.org/ N/A Other Zeiss LSM 780 Zeiss N/A Fitotron SGC 120 Growth chamber Weiss Technik, UK N/A Zeiss Axio Imager A2 Zeiss N/A 7500 Fast Real-Time PCR system Applied Biosystems N/A Zen software Zeiss N/A
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